Important practical issues covered are read-length, speed, accuracy, throughput, cost, as well as the automation of sample handling and preparation. The four best known DNA sequencing techniques are reviewed. Single molecule sequencing with exonuclease 190 ![]() Pyrosequencing – DNA sequencing in real time by the detection of released PPi 187Ħ. Maxam & Gilbert and other chemical methods 183ĥ. ![]() ![]() Sanger's method and other enzymic methods 170ģ.6.2 Mass spectrometry – an alternative 182Ĥ.
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